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APIS ESR1 qPCR Kit (RUO*)

03202

The APIS ESR1 qPCR Kit is an advanced qPCR-based assay designed for the sensitive and precise detection of mutations in the oestrogen receptor (ESR1) gene. This qualitative test identifies eleven ESR1 mutations across three exons: exon 5 (E380Q), exon 7 (S463P), and exon 8 (P535H, L536R, L536Q, L536H, L536P, Y537C, Y537S, Y537N, and D538G).

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*For Research Use Only (RUO), not for use in diagnostic procedures, developed by APIS and distributed by Biocartis NV. Data and conclusions have not been validated by Biocartis NV.

SPECIMEN REQUIREMENTS

  • 20 µl of DNA, e.g., extracted cell-free DNA (cfDNA).
  • It is recommended to extract samples using a cell-free-specific kit and to use the sample without dilution.

Benefits

  1. Wide target coverage • designed to cover 11 ESR1 mutations
  2. High sensitivity  all mutations are detected at ≤ 0.5% MAF (Mutant Allele Frequency)
  3. Instrument agnostic compatible with a variety of real-time PCR instruments

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